
Contact
Biomedical Research Center251 Bayview Boulevard
Suite 200
Room 06A719
Baltimore, MD 21224
Phone: 443-740-2585
Email: bhope@mail.nih.gov
Education
Post-doctoral Training – Laboratory of Molecular Psychiatry, Department of Psychiatry, Yale University (advisor: Dr. Eric Nestler)
Ph.D. – Neurological Research, Department of Psychiatry, University of British Columbia, Vancouver, Canada (thesis advisor: Dr. Steven R. Vincent)
B.Sc. – Biochemistry, University of British Columbia, Vancouver, Canada
Research Interests
When using drugs of abuse, learned associations are formed between the drugs and stimuli present in the drug-taking environment. With continued use, these stimuli can become cues that promote drug relapse. Our research is focused on figuring out how these memories are stored in the brain. We have identified sparsely distributed patterns of neurons in the brain called ‘neuronal ensembles’ that are selectively activated by drug-related cues and thought to encode the learned associations that mediate drug seeking behavior. Drug-related cues activate specific genes such as c-fos within these neuronal ensembles and allow us to identify them in the brain. We exploit the c-fos promoter to turn on different transgenes in transgenic rats that allow us to manipulate specific neuronal ensembles and assess their role in drug-related memories. We also developed a fluorescence-activated cell sorting (FACS) procedure for purifying these activated ensembles and found unique molecular alterations within their cell bodies and synapses. We have developed novel c-fos-GFP transgenic rats that produce green fluorescent protein (GFP) in activated neurons and found unique synaptic alterations using slice electrophysiology. Using a combination of novel viruses and transgenic rats developed in collaboration with Dr. Brandon Harvey, we continue to search and characterize drug-related memory engrams in the brain that promote drug relapse.
Publications
Selected Publications
Silent synapses in selectively activated nucleus accumbens neurons following cocaine sensitization. Journal Article In: Nat Neurosci, vol. 15, no. 11, pp. 1556–1562, 2012, ISSN: 1546-1726 (Electronic); 1097-6256 (Linking). FACS purification of immunolabeled cell types from adult rat brain. Journal Article In: J Neurosci Methods, vol. 203, no. 1, pp. 10–18, 2012, ISSN: 1872-678X (Electronic); 0165-0270 (Linking). Ventral medial prefrontal cortex neuronal ensembles mediate context-induced relapse to heroin. Journal Article In: Nat Neurosci, vol. 14, no. 4, pp. 420–422, 2011, ISSN: 1546-1726 (Electronic); 1097-6256 (Linking). FACS identifies unique cocaine-induced gene regulation in selectively activated adult striatal neurons. Journal Article In: J Neurosci, vol. 31, no. 11, pp. 4251–4259, 2011, ISSN: 1529-2401 (Electronic); 0270-6474 (Linking). Context-specific modulation of cocaine-induced locomotor sensitization and ERK and CREB phosphorylation in the rat nucleus accumbens. Journal Article In: Eur J Neurosci, vol. 30, no. 10, pp. 1931–1940, 2009, ISSN: 1460-9568 (Electronic); 0953-816X (Linking). Targeted disruption of cocaine-activated nucleus accumbens neurons prevents context-specific sensitization. Journal Article In: Nat Neurosci, vol. 12, no. 8, pp. 1069–1073, 2009, ISSN: 1546-1726 (Electronic); 1097-6256 (Linking). Context-specific sensitization of cocaine-induced locomotor activity and associated neuronal ensembles in rat nucleus accumbens. Journal Article In: Eur J Neurosci, vol. 27, no. 1, pp. 202–212, 2008, ISSN: 1460-9568 (Electronic); 0953-816X (Linking).2012
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