Cell-specific CRISPR/Cas9 in the adult rat brain.
Microscopic images of the midbrain of a transgenic rat that selectively expresses Cas9 in dopamine neurons. On the left side, control gRNAs were delivered to cells in the midbrain using a virus (green). On the right side, gRNAs to a target gene (white) is expressed in both dopaminergic neurons (red) and non-dopaminergic neurons. Arrows identify dopaminergic cells that received gRNAs. Only on the right side which received gRNAs to target (white) do we see the selective loss of target gene expression from dopaminergic neurons. Modified from Bäck, Necarsulmer et al Neuron 2019 with permission from Elsevier.
Neuron, 102 (1), pp. 105–119, 2019, ISSN: 1097-4199 (Electronic); 0896-6273 (Linking).