Transgenic mice for in vivo epigenome editing with CRISPR-based systems. Nature Methods.
Gemberling et al. generated and validated two new Cre-dependent transgenic mouse lines harboring LSL-dCas9-p300 (gene activator/histone acetyltransferase) and LSL-dCas9-KRAB (gene repressor/histone methyltransferase). The authors demonstrated that guide RNAs targeting gene enhancers and the gene promoter are effective at modulating both histone modification state and gene transcription. [Read More]