Engineering of NEMO as calcium indicators with large dynamics and high sensitivity. Nat Methods.
Current genetically encoded calcium indicators have suboptimal dynamic range and peak signal-to-baseline ratios. Li et al. engineered a new series of mNG-based calcium indicators (NEMO) that exhibit fast kinetics with up to 100-fold increase in dynamic range and 20-fold increase in signal-to-baseline ratios when NEMO sensors were compared to GCaMP6m or NCaMP7. Increased fluorescence and dynamic range of NEMO should facilitate the in vivo monitoring of calcium changes in deep brain structures and cellular organelles. [Read More]